Publications
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Preprint
Highly efficient transgenic mouse production using piggyBac and its application to rapid phenotyping at the founder generation. Okamura E, Mizuno S, Matsumoto S, Murata K, Tanimoto Y, Tra DTH, Suzuki H, Kang W, Ema T, Morimoto K, Kato K, Matsumoto T, Masuyama N, Kijima Y, Morimura T, Sugiyama F, Takahashi S, Mizutani E, Woltjen K, Yachie N, Ema M. (2024) bioRxiv, doi: 10.1101/2023.12.10.570953. [Link]
2024
Enrichment of Allelic Editing Outcomes by Prime Editing in Induced Pluripotent Stem Cells. Niwa R, Matsumoto T, Liu AY, Kawato M, Kondo T, Tsukita K, Gee P, Inoue H, Maurissen TL, Woltjen K. (2024) CRISPR Journal, 7(5), doi: 10.1089/crispr.2024.0028. [Link]
Modeling mutation‑specific arrhythmogenic phenotypes in isogenic human iPSC‑derived cardiac tissues. Maurissen TL, Kawatou M, López‑Dávila V, Minatoya K, Yamashita JK, Woltjen K. (2024) Scientific Reports, doi: 10.1038/s41598-024-52871-1. [Link]
2023
Hypoblast from human pluripotent stem cells regulates epiblast development. Okubo T, Rivron N, Kabata M, Masaki H, Kishimoto K, Semi K, Nakajima-Koyama M, Kunitomi H, Kaswandy B, Sato H, Nakauchi H, Woltjen K, Saitou M, Sasaki E, Yamamoto T, Takashima Y. (2023) Nature, doi: 10.1038/s41586-023-06871-2.
ACE2 knockout hinders SARS-CoV-2 propagation in iPS cell-derived airway and alveolar epithelial cells. Niwa R., Sakai K., Lung M.S.Y., Matsumoto T., Mikawa R., Maehana S., Suzuki M., Yamamoto Y., Maurissen T.L., Hirabayashi A., Noda T., Kubo M., Gotoh S., Woltjen K. (2023) Frontiers in Cell and Developmental Biology, 11, doi: 10.3389/fcell.2023.1290876.
mRNA-based generation of marmoset PGCLCs capable of differentiation into gonocyte-like cells. Kubiura-Ichimaru, M., Penfold, C., Kojima, K., Dollet, C., Yabukami, H., Semi, K., Takashima, Y., Boroviak, T., Kawaji, H., Woltjen, K., Minoda, A., Sasaki, E. and Watanabe, T. (2023) Stem Cell Reports, 18, 1987-2002. doi: 10.1016/j.stemcr.2023.08.006.
A disease-specific iPS cell resource for studying rare and intractable diseases. Saito, M.K., Osawa, M., Tsuchida, N., Shiraishi, K., Niwa, A., Woltjen, K., Asaka, I., Ogata, K., Ito, S., Kobayashi, S. and Yamanaka, S. (2023) Inflamm Regen., 43, 43. doi: 10.1186/s41232-023-00294-2.
Uniform transgene activation in Tet-On systems depends on sustained rtTA expression. Otomo, J., Woltjen, K. and Sakurai, H. (2023) iScience, 26, 107685. doi: 10.1016/j.isci.2023.107685.
Programmable mammalian translational modulators by CRISPR-associated proteins. Kawasaki, S., Ono, H., Hirosawa, M., Kuwabara, T., Sumi, S., Lee, S., Woltjen, K. and Saito, H. (2023) Nat Commun., 14, 2243. doi: 10.1038/s41467-023-37540-7.
Novel Calmodulin Variant p.E46K Associated With Severe Catecholaminergic Polymorphic Ventricular Tachycardia Produces Robust Arrhythmogenicity in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Gao, J., Makiyama, T., Yamamoto, Y., Kobayashi, T., Aoki, H., Maurissen, T.L., Wuriyanghai, Y., Kashiwa, A., Imamura, T., Aizawa, T., Huang, H., Kohjitani, H., Nishikawa, M., Chonabayashi, K., Fukuyama, M., Manabe, H., Nakau, K., Wada, T., Kato, K., Toyoda, F., Yoshida, Y., Makita, N., Woltjen, K., Ohno, S., Kurebayashi, N., Murayama, T., Sakurai, T., Horie, M. and Kimura, T. (2023) Circ Arrhythm Electrophysiol., 16, e011387. doi: 10.1161/CIRCEP.122.011387. Epub 2023 Mar 3.
Optimization of the proliferation and persistency of CAR T cells derived from human induced pluripotent stem cells. Ueda, T., Shiina, S., Iriguchi, S., Terakura, S., Kawai, Y., Kabai, R., Sakamoto, S., Watanabe, A., Ohara, K., Wang, B., Xu, H., Minagawa, A., Hotta, A., Woltjen, K., Uemura, Y., Kodama, Y., Seno, H., Nakatsura, T., Tamada, K. and Kaneko, S. (2023) Nat Biomed Eng., 7, 24-37. doi: 10.1038/s41551-022-00969-0. Epub 2022 Dec 12.
Disrupted CaV1.2 selectivity causes overlapping long QT and Brugada syndrome phenotypes in the CACNA1C-E1115K iPS cell model. Kashiwa, A., Makiyama, T., Kohjitani, H., Maurissen, T.L., Ishikawa, T., Yamamoto, Y., Wuriyanghai, Y., Gao, J., Huang, H., Imamura, T., Aizawa, T., Nishikawa, M., Chonabayashi, K., Mishima, H., Ohno, S., Toyoda, F., Sato, S., Yoshiura, KI., Takahashi, K., Yoshida, Y., Woltjen, K., Horie, M., Makita, N. and Kimura, T. (2023) Heart Rhythm, 20, 89-99. doi: 10.1016/j.hrthm.2022.08.021. Epub 2022 Aug 23.
2022
The oncogene-dependent resistance to reprogramming unveils cancer therapeutic targets. Ito, K., Nagata, K., Ohta, S., Matsuda, Y., Ukai, T., Yasuda, I., Ota, A., Kobayashi, R., Kabata, M., Sankoda, N., Maeda, T., Woltjen, K., Yang, L., Maruyama, R., Katayama, R., Yamamoto, T. and Yamada, Y. (2022) Cell Reports, 39, 110721.
A stress-reduced passaging technique improves the viability of human pluripotent cells. Takahashi, K., Okubo, C., Nakamura, M., Iwasaki, M., Kawahara, Y., Tabata, T., Miyamoto, Y., Woltjen, K. and Yamanaka, S. (2022) Cell Reports Methods, 2, 2.
2021
DMRT1-mediated reprogramming drives development of cancer resembling human germ cell tumors with features of totipotency. Taguchi, J., Shibata, H., Kabata, M., Kato, M., Fukuda, K., Tanaka, A., Ohta, S., Ukai, T., Mitsunaga, K., Yamada, Y., Nagaoka, S.I., Yamazawa, S., Ohnishi, K., Woltjen, K., Ushiku, T., Ozawa, M., Saitou, M., Shinkai, Y., Yamamoto, T. and Yamada, Y. (2021) Nature communications, 12, 5041.
Capturing Human Trophoblast Development with Naive Pluripotent Stem Cells in Vitro. Io, S., Kabata, M., Iemura, Y., Semi, K., Morone, N., Minagawa, A., Wang, B., Okamoto, I., Nakamura, T., Kojima, Y., Iwatani, C., Tsuchiya, H., Kaswandy, B., Kondoh, E., Kaneko, S., Woltjen, K., Saitou, M., Yamamoto, T., Mandai, M. and Takashima, Y. (2021) Cell Stem Cell.
Epithelial Expression of Gata4 and Sox2 Regulates Specification of the Squamous-Columnar Junction via MAPK/ERK Signaling in Mice. Sankoda, N., Tanabe, W., Tanaka, A., Shibata, H., Woltjen, K., Chiba, T., Haga, H., Sakai, Y., Mandai, M., Yamamoto, T., Yamada, Y., Uemoto, S. and Kawaguchi, Y. (2021) Nature communications, 12, 560.
2020
Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation. Maurissen, T.L. and Woltjen, K. (2020) Nature communications, 11, 2876.
Recapitulating the Human Segmentation Clock with Pluripotent Stem Cells. Matsuda, M., Yamanaka, Y., Uemura, M., Osawa, M., Saito, M.K., Nagahashi, A., Nishio, M., Guo, L., Ikegawa, S., Sakurai, S., Kihara, S., Maurissen, T.L., Nakamura, M., Matsumoto, T., Yoshitomi, H., Ikeya, M., Kawakami, N., Yamamoto, T., Woltjen, K., Ebisuya, M., Toguchida, J. and Alev, C. (2020) Nature, 580, 124–129.
N-Terminal Amino Acids Determine KLF4 Protein Stability in 2A Peptide-Linked Polycistronic Reprogramming Constructs. Reinhardt, A., Kagawa, H. and Woltjen, K. (2020) Stem Cell Reports, 14, 520–527.
2019
Genome-Wide Microhomologies Enable Precise Template-Free Editing of Biologically Relevant Deletion Mutations. Grajcarek, J., Monlong, J., Nishinaka-Arai, Y., Nakamura, M., Nagai, M., Matsuo, S., Lougheed, D., Sakurai, H., Saito, M.K., Bourque, G. and Woltjen, K. (2019) Nature communications, 10, 4856.
Metalloprotease-Dependent Attenuation of BMP Signaling Restricts Cardiac Neural Crest Cell Fate. Arai, H.N., Sato, F., Yamamoto, T., Woltjen, K., Kiyonari, H., Yoshimoto, Y., Shukunami, C., Akiyama, H., Kist, R. and Sehara-Fujisawa, A. (2019) Cell Reports, 29, 603–616.
Cell-Type Dependent Enhancer Binding of the EWS/ATF1 Fusion Gene in Clear Cell Sarcomas. Komura, S., Ito, K., Ohta, S., Ukai, T., Kabata, M., Itakura, F., Semi, K., Matsuda, Y., Hashimoto, K., Shibata, H., Sone, M., Jo, N., Sekiguchi, K., Ohno, T., Akiyama, H., Shimizu, K., Woltjen, K., Ozawa, M., Toguchida, J., Yamamoto, T. and Yamada, Y. (2019) Nature communications, 10, 3999.
2018
OVOL1 Influences the Determination and Expansion of iPSC Reprogramming Intermediates. Kagawa, H., Shimamoto, R., Kim, S.-I., Oceguera-Yanez, F., Yamamoto, T., Schroeder, T. and Woltjen, K. (2018) Stem Cell Reports, 12, 319–332.
Platforms of in Vivo Genome Editing with Inducible Cas9 for Advanced Cancer Modeling. Jo, N., Sogabe, Y., Yamada, Y., Ukai, T., Kagawa, H., Mitsunaga, K., Woltjen, K. and Yamada, Y. (2018) Cancer science, 110, 926–938.
Microhomology-Assisted Scarless Genome Editing in Human iPSCs. Kim, S.-I., Matsumoto, T., Kagawa, H., Nakamura, M., Hirohata, R., Ueno, A., Ohishi, M., Sakuma, T., Soga, T., Yamamoto, T. and Woltjen, K. (2018) Nature communications, 9, 939.
Press releases: CiRA Website (日本語 or English), and EurekAlert!
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In Vivo Reprogramming Drives Kras-Induced Cancer Development. Shibata, H., Komura, S., Yamada, Y., Sankoda, N., Tanaka, A., Ukai, T., Kabata, M., Sakurai, S., Kuze, B., Woltjen, K., Haga, H., Ito, Y., Kawaguchi, Y., Yamamoto, T. and Yamada, Y. (2018) Nature communications, 9, 1–16
Srf Destabilizes Cellular Identity by Suppressing Cell- Type-Specific Gene Expression Programs. Ikeda, T., Hikichi, T., Miura, H., Shibata, H., Mitsunaga, K., Yamada, Y., Woltjen, K., Miyamoto, K., Hiratani, I., Yamada, Y., Hotta, A., Yamamoto, T., Okita, K. and Masui, S. (2018) Nature communications, 1–15.
2017
Evolutionarily Distinctive Transcriptional and Signaling Programs Drive Human Germ Cell Lineage Specification From Pluripotent Stem Cells. Kojima, Y., Sasaki, K., Yokobayashi, S., Sakai, Y., Nakamura, T., Yabuta, Y., Nakaki, F., Nagaoka, S., Woltjen, K., Hotta, A., Yamamoto, T. and Saitou, M. (2017) Cell Stem Cell, 21, 517–532.e5.
Cell-Type-Specific Genome Editing with a microRNA-Responsive CRISPR-Cas9 Switch. Hirosawa, M., Fujita, Y., Parr, C.J.C., Hayashi, K., Kashida, S., Hotta, A., Woltjen, K. and Saito, H. (2017) Nucleic Acids Research, 45, e118.
The Src/C-Abl Pathway Is a Potential Therapeutic Target in Amyotrophic Lateral Sclerosis. Imamura, K., Izumi, Y., Watanabe, A., Tsukita, K., Woltjen, K., Yamamoto, T., Hotta, A., Kondo, T., Kitaoka, S., Ohta, A., Tanaka, A., Watanabe, D., Morita, M., Takuma, H., Tamaoka, A., Kunath, T., Wray, S., Furuya, H., Era, T., Makioka, K., Okamoto, K., Fujisawa, T., Nishitoh, H., Homma, K., Ichijo, H., Julien, J.-P., Obata, N., Hosokawa, M., Akiyama, H., Kaneko, S., Ayaki, T., Ito, H., Kaji, R., Takahashi, R., Yamanaka, S. and Inoue, H. (2017) Science Translational Medicine, 9 (391).
Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency. Sone, M., Morone, N., Nakamura, T., Tanaka, A., Okita, K., Woltjen, K., Nakagawa, M., Heuser, J.E., Yamada, Y., Yamanaka, S. and Yamamoto, T. (2017) Cell Metabolism, 25, 1103–1117.e6.
Cellular Context-Dependent Consequences of Apc Mutations on Gene Regulation and Cellular Behavior. Hashimoto, K., Yamada, Y., Semi, K., Yagi, M., Tanaka, A., Itakura, F., Aoki, H., Kunisada, T., Woltjen, K., Haga, H., Sakai, Y., Yamamoto, T. and Yamada, Y. (2017) Proceedings of the National Academy of Sciences, 114, 758–763.
2016
An EWS-FLI1-Induced Osteosarcoma Model Unveiled a Crucial Role of Impaired Osteogenic Differentiation on Osteosarcoma Development. Komura, S., Semi, K., Itakura, F., Shibata, H., Ohno, T., Hotta, A., Woltjen, K., Yamamoto, T., Akiyama, H. and Yamada, Y. (2016) Stem Cell Reports, 6, 592–606.
Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives. Oceguera-Yanez, F., Kim, S-I., Matsumoto, T., Tan, G. W., Xiang, L., Hatani, T., Kondo, T., Ikeya, M., Yoshida, Y., Inoue, H. and Woltjen, K. (2016). Methods. (San Diego, Calif).
2015
KLF4 N-Terminal Variance Modulates Induced Reprogramming to Pluripotency. Kim S-I, Oceguera-Yanez F., Hirohata R., Linker S., Okita K., Yamada Y., Yamamoto T., Yamanaka S. and Woltjen K. (2015). Stem Cell Reports. 4, 1-17.
Highlighted by Faculty of 1000
See the press release on the CiRA Website (日本語 or English), and on EurekAlert!
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Reprogramming Roadblocks Are System Dependent. Chantzoura, E., Skylaki, S., Menendez, S., Kim, S.-I., Johnsson, A., Linnarsson, S., Woltjen, K., Chambers, I. and Kaji, K. (2015). Stem Cell Reports. 5(3), 350–364.
Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells. Sasaki, K., Yokobayashi, S., Nakamura, T., Okamoto, I., Yabuta, Y., Kurimoto, K., Ohta, H., Moritoki, Y., Iwatani, C., Tsuchiya, H., Nakamura, S., Sekiguchi, K., Sakuma, T., Yamamoto, T., Mori, T., Woltjen, K., Nakagawa, M., Yamamoto, T., Takahashi, K., Yamanaka, S. and Saitou, M. (2015). Cell Stem Cell. 17(2), 178–194.
Mutant IDH1 Dysregulates the Differentiation of Mesenchymal Stem Cells in Association with Gene-Specific Histone Modifications to Cartilage- and Bone-Related Genes. Jin, Y., Elalaf, H., Watanabe, M., Tamaki, S., Hineno, S., Matsunaga, K., Woltjen, K., Kobayashi, Y., Nagata, S., Ikeya, M., Kato, T., Okamoto, T., Matsuda, S. and Toguchida J. (2015). PLoS ONE. 10(7), e0131998.
SS18-SSX, the Oncogenic Fusion Protein in Synovial Sarcoma, Is a Cellular Context-Dependent Epigenetic Modifier. Tamaki, S., Fukuta, M., Sekiguchi, K., Jin, Y., Nagata, S., Hayakawa, K., Hineno, S., Okamoto, T., Watanabe, M., Woltjen, K., Ikeya, M., Kato, T. and Toguchida, J. (2015). PLoS ONE. 10(11), e0142991.
Synthetic lateral inhibition governs cell-type bifurcation with robust ratios. Matsuda M., Koga M., Woltjen K., Nishida E., and Ebisuya M. (2015). Nature Communications. 6, 6195.
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2014
Premature termination of reprogramming in vivo leads to cancer development through altered epigenetic regulation. Ohnishi K., Semi K., Yamamoto T., Shimizu M., Tanaka A., Mitsunaga K., Okita K., Osafune K., Arioka Y., Maeda T., Soejima H., Moriwaki H., Yamanaka S., Woltjen K., and Yamada Y. (2014). Cell. 156(4), 663-677.
Discussed in a Cell Stem Cell Preview by Jose Polo
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2013
Transient maternal IL-6 mediates long-lasting changes in neural stem cell pools by deregulating an endogenous self-renewal pathway. Gallagher D., Norman A. A., Woodard C. L., Yang G., Gauthier-Fisher A., Fujitani M., Vessey J.P., Cancino G.I., Sachewsky N., Woltjen K., Fatt M.P., Morshead C.M., Kaplan D.R. and Miller F.D. (2013). Cell Stem Cell. 13(5), 564-576.
Discussed in a Cell Stem Cell Preview by Elizabeth Gould
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Cartilage tissue engineering identifies abnormal human induced pluripotent stem cells. Yamashita A., Liu S., Woltjen K., Thomas B., Meng G., Hotta A., Takahashi K., Ellis J., Yamanaka S. and Rancourt D.E. (2013). Scientific Reports. 3(1978).
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Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro. Tanaka A., Woltjen K., Miyake K., Hotta A., Ikeya M., Yamamoto T., Nishino T., Shoji E., Sehara-Fujisawa A., Manabe Y., Fujii N., Hanaoka K., Era T., Yamashita S., Isobe K., Kimura E. and Sakurai H.. (2013). PLoS One. 8(4), e61540.
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Efficient TALEN construction and evaluation methods for human cell and animal applications. Sakuma T., Hosoi S., Woltjen K., Suzuki K.I., Kashiwagi K, Wada H., Ochiai H., Miyamoto T., Kawai N., Sasakura Y., Matsuura S., Okada Y., Kawahara A., Hayashi S. and Yamamoto T. (2013). Genes to Cells. 18(4), 315-326.
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Identification of target genes of synovial sarcoma-associated fusion oncoprotein using human pluripotent stem cells. Hayakawa, K., Ikeya, M., Fukuta, M., Woltjen K., Tamaki, S., Takahara, N., Kato, T., Sato, S., Otsuka, T. and Toguchida, J. (2013). Biochem. Biophys. Research Communications. 432(4), 713-719.
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2012
Highly efficient site-specific transgenesis in cancer cell lines. Michael, I.P., Monetti, C., Chiu, A.C., Zhang, P., Baba, T., Nishino, K., Agha-Mohammadi, S., Woltjen K., Sung, H.K. and Nagy, A. (2012). Molecular Cancer. 11(1), 89-100.
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2011
An alternative splicing switch regulates embryonic stem cell pluripotency and reprogramming. Gabut, M., Samavarchi-Tehrani, P., Wang, X., Slobodeniuc, V., O’Hanlon, D., Sung, H-K., Alvarez, M., Talukder, S., Pan, Q., Mazzoni, E.O., Nedelec, S., Wichterle, H., Woltjen K., Hughes, T.R., Zandstra, P.W., Nagy, A., Wrana, J.L., Blencowe, B.J. (2011). Cell. 147(1), 132-146.
Article Selected for a 2012 Lap-Chee Tsui Publication Award from the CIHR Institute of Genetics
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Induced Pluripotent Stem Cell Lines Derived from Equine Fibroblasts. Nagy, K., Sung, H-K., Zhang, P., Laflamme, S., Vincent, P., Agha-Mohammadi, S., Woltjen K., Monetti, C., Michael, I.P., Hussein, S., Smith, L.C., and Nagy, A. (2011). Stem Cell Reviews and Reports. 7(3), 693-702.
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PhiC31 integrase facilitates genetic approaches combining multiple recombinases. Monetti, C., Nishino, K., Biechele, S., Zhang, P., Baba, T., Woltjen K. and Nagy, A. (2011). Methods. 53(4), 380-385.
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2010
Functional Genomics Reveals a BMP Driven Mesenchymal-to-Epithelial Transition in the Initiation of Somatic Cell Reprogramming. Samavarchi-Tehrani, P., Golipour, A., David, L., Sung, H-K., Beyer, T.A., Datti, A., Woltjen K., Nagy, A., and Wrana, J.L. (2010). Cell Stem Cell. 7, 64-77.
Discussed in a Nature Methods News and Views by Konrad Hochedlinger
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2009 and prior
Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Kaji, K., Norrby, K., Paca, A., Mileikovsky, M., Mohseni, P., and Woltjen K. (2009). Nature. 458, 771-775.
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piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.Woltjen K., Michael, I.P., Mohseni, P., Desai, R., Mileikovsky, M., Hämäläinen, R., Cowling, R., Wang, W., Liu, P., Gertsenstein, M., Kaji, K., Sung, HK., and Nagy A. (2009). Nature. 458, 766-770.
Highlighted in Nature Methods’ News and Views
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Deep screening of recombination proficient bacteriophage libraries. Thomas, B., Woltjen K., and Rancourt, D.E. (2003). Biotechniques. 34, 36-38, 40.
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Bacteriophage gene targeting vectors generated by transplacement. Aoyama, C., Woltjen K., Mansergh, F.C., Ishidate, K., and Rancourt, D.E. (2002). Biotechniques. 33, 806-810, 812.
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Retro-recombination screening of a mouse embryonic stem cell genomic library. Woltjen K., Bain, G., and Rancourt, D.E. (2000). Nucleic Acids Research. 28, E41.
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111
On the edge of deletion: Using natural and engineered microhomology to edit the human genome. Martínez-Gálvez G., Lee S., Niwa R., and Woltjen, K. (2024) Gene and Genome Editing.
Differentiation of pluripotent stem cells for modeling human skin development and potential applications. Oceguera-Yanez, F., Avila-Robinson, A. and Woltjen, K. (2022) Frontiers in Cell and Developmental Biology.
Editorial Overview: Embroidering the Canvas of Life. Woltjen, K. and Bortvin, A. (2018) Current opinion in genetics & development, 52, ii–iv.
Beta Cell Replacement Strategies for Diabetes. Kieffer, T.J., Woltjen, K., Osafune, K., Yabe, D. and Inagaki, N. (2017) Journal of Diabetes Investigation.
At the Conflux of Human Genome Engineering and Induced Pluripotency. Woltjen, K., Oceguera-Yanez, F., Kagawa, H. and Kim, S.-I. (2016) Genome Editing, Springer International Publishing, Cham, 45–64.
Nuclease-mediated genome editing: At the front-line of functional genomics technology. Sakuma T. and Woltjen K. (2014). Development, Growth and Differentiation. 56, 2-13.
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Reprogramming: The Next Generation. Hochedlinger, K., Takahashi, K., Plath, K., Ng, H.H., Clark, A., Martin, U., Hanna, J.H., Deng, H., Silva, J.C.R., Woltjen K., Boyer, L. and Wernig, M. (2012). Cell Stem Cell. 11(6), 740–743.
SPECIAL ISSUE! Highlighting the 2013 Nobel Prize in Medicine.
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Inhibition of tgf-Beta signaling improves mouse fibroblast reprogramming. Woltjen K., and Stanford, W.L. (2009). Cell Stem Cell. 5, 457-458.
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New strategies to generate induced pluripotent stem cells. O’Malley, J., Woltjen K., and Kaji, K. (2009). Current Opinion in Biotechnology. 20, 516-521.
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111
ClinVar ─ 遺伝子型と表現型を比較しながらバリアントの病原性を解釈する. 丹羽, 諒., 井本, 逸勢. (2022) 実験医学増刊, 222-224.
日本国内で「プチ」留学する. 丹羽, 諒. (2022) 実験医学, 2325.
健常人および患者に共通して観察される欠失変異に隣接した相同配列と正確なゲノム編集における相同配列の活用. Grajcarek, J., Lee, S., and Woltjen, K. (2020) 医学のあゆみ, 273:9, 744-750. (Chapter)
ヒトiPS細胞のAAVS1遺伝子座への遺伝子組込み. Lee, S., 香川, 晴., 松本, 智., Oceguera-Yanez, F. and Woltjen, K. (2019) In: 山本, 卓. and 佐久間, 哲., Eds., ゲノム編集実験スタンダード, 実験医学別冊, 205–220.
iPS細胞を用いた正確な ゲノム編集法(MhAX法). 香川, 晴., 松本, 智., Kim, S.-I. and Woltjen, K. (2018) 実験医学, 1–8.
Report on the Conference on Transposition and Genome Engineering 2015 (TGE 2015): Advancing Cutting-Edge Genomics Technology in the Ancient City of Nara. Woltjen, K., Yamamoto, T., Kokubu, C. and Takeda, J. (2016). Genes to cells. 392–395.
Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Kim, S.-I., Oceguera-Yanez, F., Sakurai, C., Nakagawa, M., Yamanaka, S., & Woltjen, K. (2015). Methods in Molecular Biology (Clifton, NJ).
The piggyBac Transposon as a Platform Technology for Somatic Cell Reprogramming Studies in Mouse. Woltjen, K., Kim, S.-I., & Nagy, A. (2015). Methods in Molecular Biology (Clifton, NJ).
Directed Myogenic Differentiation of Human Induced Pluripotent Stem Cells. Shoji, E., Woltjen, K., & Sakurai, H. (2015). Methods in Molecular Biology (Clifton, NJ).
Transgene-free production of pluripotent stem cells using piggyBac transposons. Woltjen K., Hämäläinen, R., Kibschull, M., Mileikovsky, M., and Nagy, A. (2011). Methods in Molecular Biology. 767, 87-103.
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Multiplex Gene-Expression Assay for Human Induced Pluripotent Stem Cells. Sciabica, K., Woltjen K., Hotta, A. (2010). Genetic Engineering and Biotechnology News. 30(3).
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Orpheus recombination: a comprehensive bacteriophage system for murine targeting vector construction by transplacement. Woltjen K., Ito, K., Tsuzuki, T., and Rancourt, D.E. (2008). Methods in Molecular Biology. 435, 79-94.
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